Description:

This course will emphasize laboratory safety and procedures. Additionally, regulations that govern the biotechnology laboratory will be discussed. Biological, chemical and radiation safety will all be handled through lectures, videotapes, demonstrations and field trips. There will also be exposure to good manufacturing practices (GMP), quality assurance and control procedures (QA/QC), and OSHA and FDA regulations. 1 hr. lecture/wk.

Supplies: Refer to the instructor's course syllabus for details about any supplies that may be required.

Textbook(s): For information see - http://bookstore.jccc.net

Course Fees: NONE

Course Objectives:

Upon successful completion of this course the student should be able to:

1. Recognize the responsibilities in maintaining a safe laboratory to determine its impact on the personnel, community and environment.
2. Describe the characteristics of the various hazards in the laboratory, including their identification, proper use, storage and disposal.
3. Recognize the value and need for adequate laboratory documentation and record keeping, including procedure and safety manuals.
4. Identify the risks in the laboratory and perform a risk assessment analysis.
5. Summarize the duties of the regulatory and non-regulatory rule-making agencies that impact laboratory safety.
6. Write a Laboratory Safety manual.

Content Outline & Competencies:

I. Safe Laboratory Practices
   A. Outline good housekeeping techniques.
   B. Describe proper personal practices in the laboratory, including:
      1. Appropriate clothing and footwear.
      2. Food and beverage usage.
      3. Personal hygiene practices.
   C. Follow accepted handling practices of laboratory animals.
   D. Explain documentation and record-keeping methods.
   E. Write a laboratory safety manual to demonstrate learned methods.
 
II. Principles of Occupational Safety
   A. Follow physical, electrical and fire safety regulations.
   B. Describe the importance of signs and notices.
   C. Develop plans for facility maintenance and correction of physical
safety problems.
 
III. Safety Equipment
   A. Choose and utilize appropriate Personal Protective Equipment (PPE).
   B. Choose and utilize appropriate laboratory safety equipment,
including:
      1. Chemical fume hoods.
      2. Biological safety cabinets.
      3. Fire equipment.
 
IV. Chemical Hazards
   A. Categorize the various types of chemical hazards, their use and
handling in the laboratory including:
      1. Corrosives, irritants and similar chemicals.
      2. Toxic chemicals.
   B. Follow accepted methods for neutralization of toxic chemicals.
   C. Follow regulations and techniques for waste management.
 
V. Radioactive Chemical Hazards
   A. Categorize the various types of radioactive chemical hazards, their
use and handling in the laboratory, including:
      1. Characteristics of radioisotopes.
      2. Handling and storage techniques.
      3. Dosimetry.
   B. Describe the importance of radiation monitoring and surveillance.
   C. Explain proper record-keeping methods required by federal
regulations.
   D. Adhere to accepted methods of decontamination of radioactive
materials.
   E. Follow regulations and techniques for waste management.
 
VI. Perform Simple Risk Assessment Analysis
   A. Identify hazardous agents and their specific risks.
   B. Assess the individual susceptibility of the researchers.
   C. Review experimental procedures and their associated risks.
   D. Identify routes of entry of hazardous agents into the body.
 
VII. Regulatory Agencies for the Laboratory
   A. Recognize the role of rule-making agencies in laboratory safety,
including:
      1. Federal - OSHA, EPA, NIH, CDC, FDA, NRC, USDA.
      2. State and local.
      3. Private - UL, NSF.

Methods of Evaluation of Competencies:

Evaluation of student mastery of course competencies will be accomplished using the following methods:

Exams/Quizzes         50%-60% of grade
Laboratory Manual     40%-50% of grade


   A = 90 – 100%     
   B = 80 -  89%   
   C = 70 -  79%   
   D = 60 -  69%   
   F =  0 -  59%

Caveats:

1. Computer Literacy Expectations: Students will need basic word processing and Internet searching skills for the completion of some papers, exercises and projects.

Disabilities:

If you are a student with a disability, and if you will be requesting accommodations, it is your responsibility to contact Access Services. Access Services will recommend any appropriate accommodations to your professor and his/her director. The professor and director will identify for you which accommodations will be arranged.

JCCC provides a range of services to allow persons with disabilities to participate in educational programs and activities. If you desire support services, contact the office of Access Services for Students With Disabilities (913) 469-8500, ext. 3521 or TDD (913) 469-3885. The Access Services office is located in the Success Center on the second floor of the Student Center.